陆地棉野生种系抗黄萎病筛选及抗性机制初步研究的中期报告.docx

陆地棉野生种系抗黄萎病筛选及抗性机制初步研究的中期报告 摘要：目前，黄萎病仍然是棉花重要的病害之一，对棉花产量和质量影响极大。本研究以野生棉种系为材料，通过黄萎病菌人工侵染和药物筛选，筛选出了4个抗病棉种系：CRI12、CRI32、H7124和1403-1。进一步研究表明，这4个抗病棉种系在抗病性机制方面存在差异。其中，CRI12和CRI32表现出较高的抗病性，主要是由于其激活了相关的防御酶系统，如超氧化物歧化酶（SOD）、过氧化物酶（POD）和可溶性糖酶等，以及形成了较多的抗氧化物质。而H7124和1403-1的抗病性主要是通过直接抑制病菌的生长和繁殖来实现的，表现出较强的抗菌作用。这些结果为深入挖掘野生棉种系的抗病机制提供了初步的理论依据和技术支持。 关键词：陆地棉；黄萎病；抗性机制；抗病种系 Abstract: Fusarium wilt is still one of the important diseases affecting cotton yield and quality. In this study, four resistant cotton species, CRI12, CRI32, H7124 and 1403-1 were selected from wild cotton species through artificial inoculation of Fusarium wilt and drug screening. Further studies have shown that these four resistant cotton species have different mechanisms of resistance to the disease. Among them, CRI12 and CRI32 showed higher resistance due to the activation of related defense enzyme systems, such as superoxide dismutase (SOD), peroxidase (POD) and soluble sugar enzymes, as well as the formation of more antioxidants. The resistance of H7124 and 1403-1 was mainly achieved by directly inhibiting the growth and reproduction of the pathogen, showing strong antibacterial effects. These results provide preliminary theoretical basis and technical support for exploring the mechanisms of disease resistance in wild cotton species. Keywords: Upland cotton; Fusarium wilt; Resistance mechanism; Resistant species