《外加碳源在苯酚生物降解过程中的共代谢特征》毕业学术论文.doc

PAGE IV 摘要 本文以苯酚为研究对象，采用微生物降解的方法，在其共基质条件下，添加酵母浸膏、葡萄糖和谷氨酸钠作为外加碳源，比较它们对苯酚代谢的影响，试图在提高苯酚去除率。实验结果表明：外加葡萄糖能使菌体生长延滞期明显缩短，12h时菌液OD值达到0.5 mg/100mL，并且苯酚被彻底降解。运用正交试验研究不同外加碳源及浓度对苯酚降解的影响，结果表明：单独添加葡萄糖对苯酚降解效果最有利。 关键词：苯酚；辅助碳源；生物降解；共代谢 Abstract This research focused on the effect of different added carbon sources including yeast extract, glucose and sodium glutamate on the microbial degradation of phenol under the conditions of co-substrate, in order to improve biodegradation rate of phenol. Experimental results indicate that compared to other carbon sources, glucose could reduce the lag phase of cell growth significantly. At 12h of the experiment, OD value reached 0.5 mg/100mL, and phenol was removed completely. Orthogonal test was conducted to compare the effect of different carbon sources with different concentration on the removal of phenol. Experimental results indicate that the degradation of phenol was the best when glucose was solely added. Key words：phenol; Supplementary carbon source; biodegradation; Co-metabolism 目 录 上海师范大学本科毕业论文（设计）诚信声明………………………………….. Ⅰ 上海师范大学本科毕业论文（设计）选题登记表…………………………………Ⅱ 上海师范大学本科毕业论文（设计）指导记录表………………………..…….Ⅲ 中文摘要及关键词…………………………………………………………………Ⅳ 英文摘要及关键词…………………………………………………………………Ⅴ TOC \o 1-3 \h \z \u HYPERLINK \l _Toc352699678 0 引言 PAGEREF _Toc352699678 \h 1 HYPERLINK \l _Toc352699679 1 材料与方法 PAGEREF _Toc352699679 \h 2 HYPERLINK \l _Toc352699680 1.1培养基的准备 PAGEREF _Toc352699680 \h 2 HYPERLINK \l _Toc352699681 1.2菌液制备 PAGEREF _Toc352699681 \h 2 HYPERLINK \l _Toc352699682 1.3共降解特性研究实验 PAGEREF _Toc352699682 \h 2 HYPERLINK \l _Toc352699683 1.4外加辅助碳源正交实验 PAGEREF _Toc352699683 \h 3 HYPERLINK \l _Toc352699684 1.5最优条件验证实验 PAGEREF _Toc352699684 \h 3 HYPERLINK \l _Toc352699685 1.6测试方法 PAGEREF _Toc352699685 \h 4 HYPERLINK \l _Toc352699686 2 结果与分析 PAGEREF _Toc352699686 \h 5 HYPERLINK \l _Toc352699687 2.1三氯苯酚存在下的苯酚降解特性 PAGEREF _Toc352699687 \h 5 HYPERLINK \l _Toc352699688 2.2外加碳源对苯酚共降解效果的影响 PAGEREF _Toc352699688 \h 6 HYPERLINK \l _Toc352699689 2.