糖类对厌氧氨氧化微生物代谢的影响和新型脱氮工艺初探的开题报告.docx

糖类对厌氧氨氧化微生物代谢的影响和新型脱氮工艺初探的开题报告

Title:TheEffectofSugarsonAnaerobicAmmoniaOxidizingMicrobialMetabolismandInitialExplorationofNovelDenitrificationProcesses

Introduction:

Anaerobicammoniaoxidation(anammox)isabiologicalprocessthattakesplaceunderanaerobicconditionsandisresponsiblefortheremovalofnitrogenfromwastewater.Thisprocessiscarriedoutbyanaerobicammoniaoxidizingmicroorganisms(anammoxbacteria)thatconvertammoniumandnitritetodinitrogengas.However,anammoxisacomplexprocessthatdependsonmanyfactors,includingpH,temperature,andthepresenceofcertainsubstances,suchassugars.Sugarsarewidelypresentinwastewater,andtheirinfluenceonanammoxbacteriametabolismisnotyetclear.

Objectives:

Themainobjectiveofthisresearchistoinvestigatetheeffectofsugarsonanammoxbacteriametabolismandtoexplorethepotentialofnoveldenitrificationprocesses.Specificobjectivesare:

1.Todeterminetheoptimalconcentrationofdifferentsugarsforanammoxbacteriagrowthandmetabolism.

2.Toinvestigatetheeffectofsugarsonanammoxbacteriametabolicpathwayusingmoleculartechniques.

3.Toevaluatethepotentialofsugar-drivendenitrificationprocessesforwastewatertreatment.

Methodology:

Theresearchwillbeconductedinalaboratoryusingabatchtestapproach.Anammoxbacteriawillbeculturedunderanaerobicconditionsinthepresenceofdifferentsugars(e.g.,glucose,fructose,sucrose,etc.)atvariousconcentrations.Themicrobialactivitywillbemonitoredbymeasuringnitrogentransformationratesandbiomassgrowthusinganalyticalinstruments.Themicrobialcommunitystructureandmetabolicpathwaywillbeanalyzedusingmolecularbiologytechniquessuchasnext-generationsequencingandmetagenomicanalysis.Finally,thepotentialofsugar-drivendenitrificationprocessesforwastewatertreatmentwillbeevaluatedbydesigningandtestinganoveldenitrificationsystem.

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