antiviral activity of 3(2h)- and 6-chloro-3(2h)-isoflavenes against highly diverged, neurovirulent vaccine-derived, type2 poliovirus sewage isolates抗病毒活性的3(2 h)-和6-chloro-3(2 h)-isoflavenes高度分化,neurovirulent疫苗衍生,type2脊髓灰质炎病毒污水隔离.pdf

Antiviral Activity of 3(2H)- and 6-Chloro-3(2H)- Isoflavenes against Highly Diverged, Neurovirulent Vaccine-Derived, Type2 Poliovirus Sewage Isolates 1,2 1 1 1,2 3,4 Lester M. Shulman *, Danit Sofer , Yossi Manor , Ella Mendelson , Jean Balanant , Anna Laura Salvati5, Francis Delpeyroux3,4, Lucia Fiore5 1 Central Virology Laboratory, Public Health Services Israel Ministry of Health, Chaim Sheba Medical Center, Tel Hashomer, Israel, 2 Department of Epidemiology and Preventive Medicine, Sackler Faculty of Medicine, University of Tel Aviv, Ramat Gan, Israel, 3 Biology of Enteric Viruses, Institut Pasteur, Paris, France, 4 Institut National de ´ ´ ` la Sante et de la Recherche Medicale (INSERM) U994, Paris, France, 5 National Center for Immunobiologicals Research and Evaluation (CRIVIB), Istituto Superiore di Sanita, Viale Regina Elena, Rome, Italy Abstract Background: Substituted flavanoids interfere with uncoating of Enteroviruses including Sabin-2 polio vaccine strains. However flavanoid resistant and dependent, type-2 polio vaccine strains (minimally-diverged), emerged during in vitro infections. Between 1998–2009, highly-diverged (8 to .15%) type-2, aVDPV2s, from two unrelated persistent infections were periodically isolated from Israeli sewage. Aim: To determine whether highly evolved aVDPV s derived from persistent infections retained sensitivity to isoflavenes. 2 Methods: Sabin-2 and ten aVDPV2 isolates from two independent Israeli sources wer