响应曲面法优化桑枝多糖提取工艺.pdf

响应曲面法优化桑枝多糖提取工艺Δ 1＊ 1 1# 1，2 1 1 徐卫东 ，王佩香 ，欧阳臻 ，赵 明 ，张 磊 ，汤建明 （1.江苏大学药学院，镇江市 212013；2.江苏大学附属 医院，镇江市 212001） 中图分类号 R284.2；R282.6 文献标识码 A 文章编号 1001-0408（2011）43-4064-04 摘 要 目的：利用响应曲面法优化桑枝多糖的提取工艺。方法：以桑枝多糖得率为指标，采用单因素和响应曲面法对提取温度、 提取时间、料液比和提取次数进行考察，优选最佳提取工艺。结果：最佳提取工艺条件为提取温度100℃、料液比1∶ 20、浸提时间 2.5h 、提取2次，实际测得的多糖得率为6.15％，与模型预测值基本相符。结论：模型可较好地预测桑枝多糖的得率，响应曲面法 对桑枝多糖提取条件参数优化具有可行性。 关键词 桑枝多糖；提取；响应曲面法 Optimization of the Extraction Technology of Morus alba Polysaccharide by Response Surface Method XU Wei-dong ，WANG Pei-xiang ，OUYANG Zhen ，ZHAO Ming ，ZHANG Lei ，TANG Jian-ming （School of Pharmacy ，Jiangsu University ，Zhenjiang 212013，China ） ZHAO Ming （The Affiliated Hospital of Jiangsu University ，Zhenjiang 212001，China ） ABSTRACT OBJECTIVE ：To optimize the extraction technology of Morus alba polysaccharides by response surface method （RSM ）. METHODS ：Extraction temperature ，extraction time ，ratio of material to liquid and extraction times were investigated by single factor test and RSM using the yield of M. alba polysaccharides as index to optimize the extraction technology.RESULTS ：The optimal condition obtained from RSM was as follow ：extraction temperature 100 ℃，the ratio of material to water 1∶20，extraction time 2.5 h ，and extracting for 2 times. The yield of polysaccharides was 6.15％，which was in line with predicted value basically. CONCLUSION ：The model could well predict the yield of M. alba polysaccharides ，and RSM is feasible to optimize the extraction technology of M. alba polysaccharides. KEY WORDS Morus alba polysaccharides ；Extraction ；Response surface method 根据筛选的处方工艺，放大双黄连口崩片的制备试验，制 结果，不同批次制剂的质量基本一致，表明该处方和工艺有 备一批3000片规模的口崩片，测定其硬度、脆碎度、崩解时 较好的重现性。3批双黄连口崩片崩解分析结果见表6。