人重组pcDNA6.2生长分化因子5质粒的构建及鉴定.pdf

Journal Rehabilitative7msue Research ofClinical 9-2008V01．12，No．37 Engineering September 人重组pcDNA6．2／生长分化因子5质粒的构建及鉴定☆ 曲彦隆，武志超，王春雷，周继辉 recombinant differentiationfactor-5 Constructionandidentificationofhuman pcDNA6．2／growth plasmid Ji-hui Chun-Ioi。Zhou Qufan—long，WuZhi-chao，Wang Abstract D印artmentof allkind that and BACKGRoUND：Growthdifferentiationfactor-5is of factors stimulatethe oftendon growth may molding ligament, OrthopaecSurgery an roleontheconstructionofactivetissue tendon． inaddition．reinforcethe reaction．It important healing plays engineering theFirstClinical constructhumanrecombinant differentiationfactor一5口lasmidand abasisfor OBJECTiVE：To pcDNA6．2／growth provide inducing Hospital．Harbin Medical University,bonemarro．derivedstromalstemcellstodifferentiateintotendon． Harbin 15000I． ANDSETTING：1k trialinvitro，was in ofGenetics DESIGN，TIME experiment，cellgeneengineering completedD印删mem Heilongjiang and ofMolecular Medical from toDecemberin2007． Province，China Department Biology，HarbinUniversityAugust MATERIALS：Aof were