biotinidase is a novel marker for papillary thyroid cancer aggressivenessbiotinidase是乳头状甲状腺癌侵犯的新标志.pdf

Biotinidase is a Novel Marker for Papillary Thyroid Cancer Aggressiveness 1 1 1 1 3 Anthony K.-C. So , Jatinder Kaur , Ipshita Kak , Jasmeet Assi , Christina MacMillan , 1,2,3,4,5 . 1,2,3,4,5 . Ranju Ralhan * , Paul G. Walfish * 1 Alex and Simona Shnaider Laboratory in Molecular Oncology, Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Joseph Wolf Lebovic Health Complex, Toronto, Ontario, Canada, 2 Joseph and Mildred Sonshine Family Centre for Head and Neck Diseases, Mount Sinai Hospital, Toronto, Ontario, Canada, 3 Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Joseph Wolf Lebovic Health Complex, Toronto, Ontario, Canada, 4 Department of Otolaryngology–Head and Neck Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada, 5 Department of Otolaryngology–Head and Neck Surgery, University of Toronto, Toronto, Ontario, Canada Abstract Biotinidase was identified in secretome analysis of thyroid cancer cell lines using proteomics. The goal of the current study was to analyze the expression of biotinidase in thyroid cancer tissues and fine needle aspiration (FNA) samples to evaluate its diagnostic and prognostic potential in thyroid cancer. Immunohistochemical analysis of biotinidase was carried out in 129 papillary thyroid cancer (PTC, 34 benign thyroid tissues and 43 FNA samples and correlated with patients’ prognosis. Overall biotinidase expression was decreased in PTC compared to benign nodules (p = 0.001). Comparison of aggressive and non-aggressive PTC showed decrease in overall biotinidase expression in the former (p = 0.001). Loss of overall biotinidase expression was associated with poor disease free su