标准石英粉尘及青石棉直接诱导8羟基脱氧鸟苷加合物-环境科学学报.PDF

21 4 Vol. 21, No.4 2001 7 ACTA SCIENTIAE CIRCUMSTANTIAE July, 2001 :2001 : R9946 :A 8 袭著革, 晁福寰, 孙咏梅, 杨丹凤, 张华山, 刘中文 ( , 300050) : , DNA 8 . , DNA 8, ; H O DNA . , ; 2 2 DNA 8. : ; ; DNA ;8; Study on the formation of 8hydroxy2 deoxyguanosine adduct directly induced by standard silicon dust and crocidolite XI Zhuge, CHAO Fuhua , SUN Yo gmei, YANG Da fe g, ZHANG Huasha , LIU Zho gwe ( I stitute of health a d e viro me tal medici e, academy of military medical scie ces,Tia ji 300050) Abstract:The formatio a dmecha ism of oxidative DNA damage marker8hydroxy2deoxygua osi e, i duced by sta dard silico dust a d crocidolite i vitro, were discussed usi g high performa ce liquid chromatograph coupled with electrochemical detectio . The results showed that sta dard silico dust a d crocidolit fibers cause DNA oxidative damage to form 8hydroxy2deoxygua osi e; but the effect i duced by crocidolit fibers is sro ger tha by sta dard silico dust. The catalytic ability of H O oxidizi g DNA by sta dard silico dust is sighifica tly 2 2 differe t from tha t by crocidolit fibers. Both possece direct ge otoxicity. To i duce DNA formi g stro gly mutage tic 8hydroxy2deox ygua osi e is o e of the possible mecha ism of their pote tial carci oge sis. Keywords: sta dard silico dust; crocidolit; oxidative DNA damage; 8hydroxy2deoxygua osi e; HPLCECD ( SiO ) , , 2 [ 1] , ( IARC) , [2] . ( Na2 O!Fe2O3 !3FeO!8SiO2 !H2O) . , DNA