studies on bioflocculant production by arthrobacter sp. raats, a freshwater bacteria isolated from tyume river, south africa研究通过节细菌属sp bioflocculant生产。raats,淡水细菌隔绝tyume河,南非.pdf

Int. J. Mol. Sci. 2012, 13, 1054-1065; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Studies on Bioflocculant Production by Arthrobacter sp. Raats, a Freshwater Bacteria Isolated from Tyume River, South Africa Leonard V. Mabinya, Sekelwa Cosa, Uchechukwu Nwodo and Anthony I. Okoh * Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa; E-Mails: lmabinya@ufh.ac.za (L.V.M.); 200804075@ufh.ac.za (S.C.); 201101482@ufh.ac.za (U.N.) * Author to whom correspondence should be addressed; E-Mail: aokoh@ufh.ac.za; Tel.: +27-406-022-365; Fax: +27-866-286-824. Received: 24 November 2011; in revised form: 6 January 2012 / Accepted: 17 January 2012 / Published: 19 January 2012 Abstract: A bioflocculant-producing bacteria was isolated from Tyume River in the Eastern Cape Province, South Africa and identified by 16S rRNA gene nucleotide sequence to have 91% similarity to Arthrobacter sp. 5J12A, and the nucleotide sequence was deposited in GenBank as Arthrobacter sp. Raats (accession number HQ875723). The bacteria produced an extracellular bioflocculant when grown aerobically in a production medium containing glucose as sole carbon source and had an initial pH of 7.0. Influences of carbon, nitrogen and metal ions sources,