study of drug metabolism by xanthine oxidase由黄嘌呤氧化酶的研究药物代谢.pdf

Int. J. Mol. Sci. 2012, 13, 4873-4879; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Study of Drug Metabolism by Xanthine Oxidase Jing Zhao 1, Xiaolin He 1, Nana Yang 2, Lizhou Sun 2,* and Genxi Li 1,3,* 1 Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; E-Mails: jzhao11@ (J.Z.); hklhxl@126.com (X.H.) 2 Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China; E-Mail: nanayang210@163.com 3 Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China * Authors to whom correspondence should be addressed; E-Mails: lizhou_sun121@ (L.S.); genxili@ (G.L.); Tel.: +86-25(L.S.); +86-25(G.L.); Fax: +86-25(L.S.); +86-25(G.L.). Received: 13 January 2012; in revised form: 31 March 2012 / Accepted: 16 April 2012 / Published: 18 April 2012 Abstract: In this work, we report the studies of drug metabolism by xanthine oxidase (XOD) with electrochemical techniques. Firstly, a pair of stable, well-defined and quasi-reversible oxidation/reduction peaks is obtained with the formal potential at −413.1 mV (vs. SCE) after embedding XOD in salmon sperm DNA membrane on the surface of pyroly