耐碳青霉烯类鲍曼不动杆菌噬菌体ab3的基因组-第三军医大学学报.doc

耐碳青霉烯类鲍曼不动杆菌张 劼耐碳青霉烯类鲍曼不动杆菌EditSeq、tRNAscan-SE、TRF、FindTerm、ORF finder、BPROM、GeneMarkTM、Clustalx、phylip等软件对所获噬菌体AB3基因组的一般特性、编码基因的功能预测、RNA聚合酶基因系统的进化进行分析。结果 噬菌体AB3基因组为全长31,185bp的双链DNA，G+C mol%为39.18%，包含28个预测基因，1个转录终止子和4个可能的启动子序列。结论 基因分析和RNA聚合酶基因进化分析显示噬菌体AB3与噬菌体AB1类似，均属于phiKMV-like病毒属。 [关键词] 耐碳青霉烯类鲍曼不动杆菌Q939 [文献标识码] A Analysis of the bioinformatics of carbapenem-resistant Acinetobacter baumannii phage AB3 genome ZHANG Jie, LIU Xi , GAN Dan (Department of Geriatrics Medicine, The Third People’s Hospital of Chongqing, Chongqing 400014, China) [Abstract] Objective To sequence carbapenem-resistant Acinetobacter baumannii phage AB3 separated by the lab and analyze its bioinformatics, so as to lay a foundation of the genome remodeling and phage therapy. Methods Shot-gun library and contig package strategy were carried out for sequencing phage AB3 genome. Such software as EditSeq, tRNAscan-SE, TRF, FindTerm, ORF finder, BPROM and GeneMarkTM were applied to predict both general characteristics of the phage AB3 genome and coding gene function. In addition, the evolution of RNA polymerase gene system was analyzed with software of Clustalx and phylip. Results The genome of phage AB3 was a double-strand DNA with a full length of 31,185bp, in which G+C mol% was 39.18% and 28 predicted genes, 1 transcription terminator, and 4 possible promoter sequences were included. Conclusion Analysis of gene and RNA [基金项目重庆市卫生局科研资助项目（2009-2-081,2011-2-334 2012-2-218) [通信作者：ixj960054@163.com polymerase gene evolution indicate that phage AB3 is similar to phage AB1 and both belong to phiKMV-like virus. [Key Words] Carbapenem-resistant Acinetobacter baumannii; Phage; Genome; Bioinformatics analysis 鲍曼不动杆菌对常用的抗菌药物具有广泛的耐药性，而碳青霉烯类抗生素是目前治疗鲍曼不动杆菌感染最有效的药物之一。但随着近年来感染耐碳青霉烯类鲍曼不动杆菌 1.材料与方法 1.1 菌株和噬菌体 耐碳青霉烯类不动杆菌Phred/Phrap/Consed软件完成[4]。 1.3 噬菌体AB3基因组成分析 噬菌体AB3基因组成分析采用Lasergene软件的EditSeq软件完成；运用美国华盛顿大学编写的tRNAscan-SE软件对噬菌体AB3基因组中tRNA基因进行搜索[5]；通过串联重复查找（andem repeat finder,TRF）软件预测