甲壳素纳米纤维溶液作为内镜黏膜下注射液可行性研究 (1).pdf

临床军医杂志2024年3月第52卷第3期ClinJMed(March,2024267.

甲壳素纳米纤维溶液作为内镜黏膜下

注射液可行性研究

吕伟彦12，罗娅媛²，白燕鸥”，丁思元²，卢盛言²，刘旭？

1.锦州医科大学北部战区总医院研究生培养基地，辽宁沈阳110016；

2.北部战区总医院消化内科，辽宁沈阳110016

[摘要]探讨甲壳素纳米纤维（ChitinNFs）溶液作为内镜黏膜下注射液的可行性。方法在离体猪胃黏膜下分别注

目的按

射0.1%0.2%的ChitinNFs溶液及生理盐水，分别记为0.1%ChitinNFs组、0.2%ChitinNFs组、对照组。应用格尺在黏膜外

测量各组注射后不同时间点黏膜隆起高度。应用超声小探头收集注射后各个时间点黏膜下影像。收集注射60min后黏膜

标本进行HE染色。结果黏膜外测量及超声影像黏膜下检测发现，0.1%ChitinNFs组、0.2%ChitinNFs组黏膜下注射后各

时间点隆起高度显著高于对照组，差异有统计学意义（P0.05）。HE染色发现，0.1%ChitinNFs组、0.2%ChitinNFs组黏膜

层和固有肌层明显分离，可见注射液垫。结论ChitinNFs溶液作为内镜黏膜下注射液具有明确的可行性。

[关键词]甲壳素纳米纤维溶液；内镜；黏膜下注射；可行性

中图分类号：R318.08doi:10.16680/j.1671-3826.2024.03.12文章编号：1671-3826(2024)03-0267-05

Feasibilitystudyonchitinnanofibrilssolutionasanendoscopicsubmucosalinjection

LYUWei-yan*2,LUOYa-yuan’,BAIYan-ou?,DINGSi-yuan’,LUSheng-yan’,LIUXu?(1.GraduateTrainingBaseofGeneralHospi-

talofNorthernTheaterCommandofJinzhouMedicalUniversity,Shenyang110016,China;2.DepartmentofGastroenterology,General

HospitalofNorthernTheaterCommand,Shenyang110016,China)

Abstract:Objective

Toinvestigatethefeasibilityofusingchitinnanofibrils(ChitinNFs)solutionasasubmucosalinjection.

Methods0.1%ChitinNFssolution,0.2%ChitinNFssolutionandnormalsalinewereinjectedintothesubmucosaoftheisolatedpig

stomach,whichwererecordedas0.1%ChitinNFsgroup,0.2%ChitinNFsgroupandcontrolgroup,respectively.Themucosalprotru-

sionheightwasmeasuredoutsidethemucosausingagridatdifferenttimepointsafterinjection.Applyasmallultrasoundprobetocol-

lectsubmucosalimagesatvarioustimepointsafterinjection.CollectmucosalsamplesforHEstaining60minutesafterinjection.

ResultsExtramucosalmeasurementandsubmucousexaminationofultraso