synthesis of triptorelin lactate catalyzed by lipase in organic media由脂肪酶在有机催化合成乳酸曲普瑞林的媒体.pdf

Int. J. Mol. Sci. 2012, 13, 9971-9979; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Synthesis of Triptorelin Lactate Catalyzed by Lipase in Organic Media Hong Zhuang 1, Zhi Wang 2, Jiaxin Wang 2, Hong Zhang 2,3, Erna Xun 2, Ge Chen 2, Hong Yue 2, Ning Tang 1 and Lei Wang 2,* 1 Department of Food Science and Engineering, Jilin University, Changchun 130021, China; E-Mails: Zhuanghong@ (H.Z.); downingjlu@ (N.T.) 2 Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130021, China; E-Mails: wangzhi@ (Z.W.); jiaxin1012@163.com (J.W.); Zhanghong163163@163.com (H.Z.); xunerna86@163.com (E.X.); chengeilm4196@ (G.C.); xinqingtianlan@ (H.Y.) 3 College of Chemistry, Jilin University, Changchun 130023, China * Author to whom correspondence should be addressed; E-Mail: w_lei@; Tel.: +86-431 Fax: +86-431 Received: 23 May 2012; in revised form: 30 June 2012 / Accepted: 2 August 2012 / Published: 10 August 2012 Abstract: Triptorelin lactate was successfully synthesized by porcine pancreatic lipase (PPL) in organic solvents. The effects of acyl donor, substrate ratio, organic solvent, temperature, and water activity were investigated. Under the optimum conditions, a yield of 30% for its ester could be achieved in the reaction for about 48 h. Keywor