susceptibility towards enterotoxigenic escherichia coli f4ac diarrhea is governed by the muc13 gene in pigs磁化率对产肠毒素的大肠杆菌f4ac腹泻是由muc13基因在猪身上.pdf

Susceptibility towards Enterotoxigenic Escherichia coli F4ac Diarrhea Is Governed by the MUC13 Gene in Pigs 1 1,2 1 1 1¤ 1 1 Jun Ren *, Xueming Yan , Huashui Ai , Zhiyan Zhang , Xiang Huang , Jing Ouyang , Ming Yang , 1 1 1 1 1 1 1 Huaigu Yang , Pengfei Han , Weihong Zeng , Yijie Chen , Yuanmei Guo , Shijun Xiao , Nengshui Ding , Lusheng Huang1* 1 Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, People’s Republic of China, 2 College of Life Science, Jiangxi Science and Technology Normal University, Nanchang, People’s Republic of China Abstract Enterotoxigenic Escherichia coli (ETEC) F4ac is a major determinant of diarrhea and mortality in neonatal and young pigs. Susceptibility to ETEC F4ac is governed by the intestinal receptor specific for the bacterium and is inherited as a monogenic dominant trait. To identify the receptor gene (F4acR), we first mapped the locus to a 7.8-cM region on pig chromosome 13 using a genome scan with 194 microsatellite markers. A further scan with high density markers on chromosome 13 refined the locus to a 5.7-cM interval. Recombination breakpoint analysis defined the locus within a 2.3-Mb region. Further genome- wide mapping using 39,720 informative SNPs revealed that the most significant markers were proximal to the MUC13 gene in the 2.3-Mb region. Association studies in a collection of diverse outbred populations strongly supported that MUC13 is the most likely responsible gene. We characterized the porcine MUC13 gene that encodes two