中药白头翁乙醇提取物对小菜蛾的杀虫活性测定.docx
中药白头翁乙醇提取物对小菜蛾的杀虫活性测定
摘要:为了进一步评估中药白头翁乙醇提取物在杀虫活性方面的应用价值,本试验以小菜蛾作为供试对象,采用小叶碟添加法、微量点滴法、培养皿叶盘法,测定中药白头翁对小菜蛾的拒食、胃毒、触杀、取食选择性,明确中药白头翁在杀虫活性方面应用潜力。试验结果表明,中药白头翁对小菜蛾具有较强的拒食活性,3龄小菜蛾12、24、36、48h的AFC50值分别为47.792、37.744、24.819、19.812mg/mL;具有一定的胃毒活性,对3龄小菜蛾24h、36h、48h、60h、72h的LC50值分别为33.275、5.890、30.288、2.214、40.124mg/mL;具有一定的触杀活性对3龄小菜蛾36h、48h、60h、72h、的LC50值分别为11.42、15.097、20.760、24.402mg/mL。在取食选择性试验中,取食高浓度的叶片时虫子数量要少于取食低浓度叶片的虫子数量。
关键词:中药白头翁;杀虫活性
DeterminationofinsecticidalactivityofethanolextractoftraditionalChinesemedicinePulsatillachinensis
Absrtact:InordertofurtherevaluatetheapplicationvalueofethanolextractoftraditionalChinesemedicinePulsatillachinensisininsecticidalactivity,thisexperimenttookdiamondbackmothasthetestobject,andusedleafplateadditionmethod,microdripmethodandpetridishleafdiscmethodtodeterminethefoodrefusal,gastricpoisoning,contactkillingandfeedingselectivityofChinesemedicineLeucodonagainstdiamondbackmoth,andclarifiedtheapplicationpotentialoftraditionalChinesemedicineWhiteheadmothininsecticidalactivity.TheresultsshowedthattheAFC50valuesofWallymothat12,24,36and48hourswere47.792、37.744、24.819and19.812mg/mL,respectively.Ithascertaingastrictoxicityactivity,andtheLC50valuesof24h,36h,48h,60hand72hofthethirdinstardiamondbackmothwere33.275,5.890,and,respectively.30.288、2.214、40.124mg/mL;TheLC50valuesof36h,48h,60hand72hwere11.42,15.097,20.760and24.402mg/mL,respectively.Inthefeedingselectivitytest,thenumberofbugswhenfeedingonleaveswithhighconcentrationsislowerthanthenumberofbugseatingleaveswithlowconcentrations.
Keywords:Pulsatillachinensis;Insecticidalactivity
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目录
TOC\o1-3\h\u
1引言 2
1.1白头翁 2
1.2白头翁的药理作用 2
1.3白头翁的农药活性现状 3
1.4小菜蛾 3
1.5立题依据及选题意义 3
2材料和方法 3
2.1供试材料 3
2.1.1供试昆虫 3
2.1.2供试药剂 3
2.1.3供