中药白头翁乙醇提取物对小菜蛾的杀虫活性测定.docx

中药白头翁乙醇提取物对小菜蛾的杀虫活性测定

摘要：为了进一步评估中药白头翁乙醇提取物在杀虫活性方面的应用价值，本试验以小菜蛾作为供试对象，采用小叶碟添加法、微量点滴法、培养皿叶盘法，测定中药白头翁对小菜蛾的拒食、胃毒、触杀、取食选择性，明确中药白头翁在杀虫活性方面应用潜力。试验结果表明，中药白头翁对小菜蛾具有较强的拒食活性，3龄小菜蛾12、24、36、48h的AFC50值分别为47.792、37.744、24.819、19.812mg/mL；具有一定的胃毒活性，对3龄小菜蛾24h、36h、48h、60h、72h的LC50值分别为33.275、5.890、30.288、2.214、40.124mg/mL；具有一定的触杀活性对3龄小菜蛾36h、48h、60h、72h、的LC50值分别为11.42、15.097、20.760、24.402mg/mL。在取食选择性试验中，取食高浓度的叶片时虫子数量要少于取食低浓度叶片的虫子数量。

关键词：中药白头翁；杀虫活性

DeterminationofinsecticidalactivityofethanolextractoftraditionalChinesemedicinePulsatillachinensis

Absrtact：InordertofurtherevaluatetheapplicationvalueofethanolextractoftraditionalChinesemedicinePulsatillachinensisininsecticidalactivity,thisexperimenttookdiamondbackmothasthetestobject,andusedleafplateadditionmethod,microdripmethodandpetridishleafdiscmethodtodeterminethefoodrefusal,gastricpoisoning,contactkillingandfeedingselectivityofChinesemedicineLeucodonagainstdiamondbackmoth,andclarifiedtheapplicationpotentialoftraditionalChinesemedicineWhiteheadmothininsecticidalactivity.TheresultsshowedthattheAFC50valuesofWallymothat12,24,36and48hourswere47.792、37.744、24.819and19.812mg/mL,respectively.Ithascertaingastrictoxicityactivity,andtheLC50valuesof24h,36h,48h,60hand72hofthethirdinstardiamondbackmothwere33.275,5.890,and,respectively.30.288、2.214、40.124mg/mL；TheLC50valuesof36h,48h,60hand72hwere11.42,15.097,20.760and24.402mg/mL,respectively.Inthefeedingselectivitytest,thenumberofbugswhenfeedingonleaveswithhighconcentrationsislowerthanthenumberofbugseatingleaveswithlowconcentrations.

Keywords：Pulsatillachinensis；Insecticidalactivity

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目录

TOC\o1-3\h\u

1引言 2

1.1白头翁 2

1.2白头翁的药理作用 2

1.3白头翁的农药活性现状 3

1.4小菜蛾 3

1.5立题依据及选题意义 3

2材料和方法 3

2.1供试材料 3

2.1.1供试昆虫 3

2.1.2供试药剂 3

2.1.3供