通冠胶囊对大鼠颈动脉内皮损伤后血管VEGF eNOS表达和血清NO浓度的影响_王磊.pdf

辽宁中医杂志 2010年第 37卷第 7期 # 1393# V GF eNOS NO , , , , (广州中医药大学, 广东 广州 510120) : 目的:探讨通冠胶囊促进动脉损伤内皮修复的分子机制方法:建立大鼠左颈总动脉内皮损伤 型, 24只雄性 SD大鼠随机等分为治疗组对照组和 型组, 治疗组给予通冠胶囊水煮液( 600mg/kg) 灌胃, 对照组给予辛伐他汀( 2mg/ kg), 型组予生理盐水( 10mL /kg); 8周后,取材 H 染色和弹力纤维染色,计算机图像分析系统检测颈动脉内皮损伤处内 膜面积( intmi a area, IA)中膜面积(media area,MA)和内膜/中膜面积比( IA /MA ), 免疫组织化学法检测 V GF和 eNOS 的 表达,分光光度法测定血清NO水平结果: 治疗组对照组的 IA和 IA /MA 均小于 型组(P 01 01), 治疗组对照组血管 内膜V GFeNOS表达和血清NO水平与均高于 型组 (P 01 05或P 01 01), 治疗组的血清 NO水平高于对照组(P 0105)结论: 通冠胶囊促进损伤内皮修复的分子机制可能与V GF- AKT- eNOS- NO信号转导通路有关 : 通冠胶囊; 内皮损伤;血管内皮生长因子; 内皮型一氧化氮合成酶; 一氧化氮; 大鼠 : R28515 : A : 1000- 1719( 2010) 07- 1393- 03 Effects of Tongguan Capsu le on the Expression of VEGF eNOS in Intim a and Serum Level of NO in R ats after Carotid Endothe lium In ury WAN G L e,i ZHANG M in-zhou, ZI Yong, H UAN G J ian-an, CH EN P eng (Guang dong H osp ita of Traditiona Ch inese M ed ic ine, Guangzhou 510120, Ch ian ) Abstrac t: O bj ectiv e: To investigate the molecular mechanism ofTongguan Capsule accelerating the recovery of arterial endo- thelium injury.M ethods: 24 male Sprague- Daw ley ratswhich the leftcommon carotid arteries injuredwere randomly divided into3 - 1 - 1 - 1 - 1 groups( n = 18): Group 1( 10mg# kg # d ofPS w as given after injury), group 2( 2mg# kg # d of Smi vastatin was given after injury) and group 3( 600mg/kg/d ofTongguan capsulew as given after injury) . A fter 8w eek, the injured left common carotid arteriesw ere removed