土鳖虫水煎剂对人多囊肾病囊肿衬里上皮细胞增殖的影响_徐成钢.pdf

200 Acad J Sec Mil Med Univ2002 Feb; 23( 2) 徐成钢, 梅长林, 赵海丹　( , 200003) [ ] 目的: 方法: SD , 1640 : SD RPMI ( 1) (EGF) 02. 551020 ng/ ml; ( 2) 1% 2. 5% 5% , ; ( 3) EGF 10 ng/ ml 1% 2. 5% 5% , EGF 48 , 5--2-( Brdu) 结果: EGF , ; , , EGF 结论: [ ] ; , , ; [ ] R 692. 12; R 282. 74 [ ] A [ ] 0258-879X(2002) 02-0200-03 Effect of eup olyp hag a s eu steleop hag a on proliferation of autosomal dominant polycystic kidney disease - cyst lining epithelial cells - , - , - ( , , XU C eng Gang MEI C ang Lin ZHAO Hai Dan Department of Nep rology C angz eng Hospital Second Military Medical University , S ang ai 200003, C ina) [ ABSTRACT] Objective: To observe t e effect of eup olyp hag a se u steleop hag a ( ESS) on t e proliferation of autosomal dominant polycyst ic kidney disease ( ADPKD) cyst -lining epit elial cells. Methods: ESS serum and control serum were . - obtained from SD rat s gastrogavaged w it ESS wat er solution and p ysiological solution ADPKD cyst lining epit elial cells w ere treated w it RPM I 1640 containing : ( 1) EGF 0, 2. 5, 5, 10, 20 ng/ ml; ( 2) ESS serum and control serum( 1% , 2. 5% , 5%) ; ( 3) EGF 10 ng/ ml, ESS serum and control serum ( 1% , 2. 5% , 5%) . T e cell proliferation was det ermined by Brdu . : - ; incorporation