多模态非线性光学显微系统.PPT

多模态非线性光学显微系统 王若曦：共焦显微技术（Confocal Microscopy） 吕卓明：二次谐波，双光子，多光子三种显微成像系统 余建：相干拉曼散射显微术(Coherent Raman Scattering Microscopy) 曾佑君：Multimodal nonlinear optical microscopy Confocal Microscopy 二次谐波显微成像的原理及装置 原理： 二次谐波产生(又称倍频)过程是一个非线性光学过 程。二次谐波产生过程可以看作是不同频率光子的交换过程。两个频率为 ω 的光子在谐波产生过程中湮灭，同时形成一个新的频率为 2ω 的光子。它是一个纯粹的量子力学过程。 多光子显微成像 多光子显微成像技术是基于双光子荧光和二次谐波产生的。 [1]庄正飞, 郭周义, 刘汉平, 喻碧英, 吴淑莲, 卓双木, 邓小元 二次谐波显微成像技术 [ J]. 激光生物学报, 2008, 17(1): 126-132. [2]VOLODYM YR N, BOAZ N. A Two photon and Second harmonic Microscope [J]. Methods, 2003, 30: 3-15. Catalog 1. Introduction 2.NLO modalities 3.Strategies in biological and biomedical science 4.Example 1.Introduction Advantage of Multimodal nonlinear optical microscopy 2.NLO modalities 3.Strategies in biological and biomedical science 4.Example Non-linear optical microscopy of kidney tumours [1]Watson J M, Marion S L, Rice P F, et al. In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis[J]. Cancer biology therapy, 2014, 15(1): 42. [2]Adur J, Carvalho H F, Cesar C L, et al. Nonlinear Optical Microscopy Signal Processing Strategies in Cancer[J]. Cancer informatics, 2014, 13: 67. [3]/doi/10.1002/lpor.201000027/abstract [4]/facilities/multimodal_nonlinear_microscopy.html [5]Jepsen P U, Cooke D G, Koch M. Terahertz spectroscopy and imaging – Modern techniques and applications[J]. Laser Photonics Reviews, 2012, 6(3). One platform, many insights! A multimodal NLO microscope, which integrates multiple imaging modalities on the same platform, allows visualization of different endogenous and exogenous structuresin cells and tissues. Coupling of nonlinear optics and scanning microscopy has generated a panel of imaging tools for biology and materials research. The nonlinear optical (NLO) microscopy family can be categorized into one-beam and two-beam modalities. The one-beam modality includes multiphoton fluorescence , second-harm