2013分子遗传学.doc

2013-2014学年第一学期农学院研究生考试卷 张金宝 王瑞雪 姓名 年级 2013 专业 动物遗传育种与繁殖 课程 动物分子数量遗传学 任课教师 冯登侦 1 .Explain term (20 point ,per term 10 point ) 1.1 genomic library Extract the entire DNA of an creature, use appropriate restriction enzymes, then, cut DNA to a range of sizes DNA fragments. And then, connect these DNA fragments separately with the carrier, which is imported the recipient bacteria group in storge. Each recipient bacteria contains a different DNA segment. That is to say, this group contains all the genes of the creature, called genomic library of this creature. 1.2 What is a QTL A QTL (quantitative trait locus) is a segment of DNA that affects a quantitative trait. We will use‘QTLs’to denote quantitative trait loci (plural), but note that in the literature the acronym‘QTL’is often used both to denote single and multiple loci. Mapping is the determination of the segment of DNA.QTL mapping is a method to locate trait genes on chromosomes. 2. solve problem( 70 point ) 2.1 Approximately how many restriction fragment would result from the complete digestion of the human genome (3×109bases)with the following restriction enzymes ?(the recongnition sequence for each enzyme is givenin parentheses). ( 20 point ) a. Sau3A( ^GATC ) b. BamHI(G^GATCC) c.SfiI(GGCCNNNN^NGGCC) a 3 × 109 / ~256 = ~fragment ~256 bp in length (44) b 3 × 109 / ~4096 = ~ 732422 fragment ~4096 bp in length (46) c 3 × 109 / = ~ 45 fragment bp in length (413) 2.2 Which of the following set(s) of primers could be used to amplify the target DNA sequence indicated by the dot？ 5’GGCTAAGATCTGAATTTTCCGAG….TTGGGCAATAATGTAGCGCCTT3’ 3’CCGATTCTAGACTTAAAAGGCTC….AACCCGTTATTACATCGCGGAA5’ a. 5’GGAAAATTCAGATCTTAG3’; 5’TGGGCAATAATGTAGCG 3’ b. 5’GCTAAGATCTGAATTTTC3’; 3’ACCCGTTATTACATCGC 5’ c. 3’GATTCTAGACTTAAAGGC5’; 3’ACCCGTTATTACATCGC 5’ d. 5’GCTAAGATCTGAATTTTC3’; 5’TGGGCAATAATGTAGCG 3’ Primer design has the following prin