同型半胱氨酸硫内酯诱导人脐静脉内皮细胞凋亡及其分子机制的研究.pdf

178 A cad J PLA Postg ad M ed Sch 2006 Jun, 27( 3 ) 谷伟军, 陆菊明, 郭清华, 杨国庆, 母义明, 汪保安, 潘长玉 ( , 100853) [ ] : ( homo cyste ine thio lactone, H cyT ) caspase3: H cyT , , RT-PCR W este n b lo t caspase3 mRNA : H cyT caspase3 mRNA ( P 0. 05) 1mm o l/L H cyT , , N- E ( 23. 16 4. 27)% ( 1. 95 0. 23)% ( 11. 01 2. 62)% ( 11. 46 1. 81)% ( P 0. 05) : H cyT , caspase3 , caspase3, [ ]; ; ; [ ] R 335 [] A [] 1005-1139( 2006) 03-0178-03 Homocysteine thiolactone induced the apoptosis ofhuman umbilicalvein endothelialcells GU W ei-j un, L U Ju-m ing, GUO Q ing-hua, YAN G Guo-Qing, M U Yi-M ing, WAN G B ao-A n, PAN Chang-Yu (D e- p artm ent o E ndocrinology, PLA G eneralH osp ital, B e ij ing 100853, Ch ina ) [ABSTRACT] Objective: T o investigate the effects of hom ocysteine th io lac tone ( H cyT ) on apopto sis and caspase3 exp ession in hum an um bilical ve in endo the lia l ce ll ( HUV EC) and study the o le of som e antiox idants ( N-acety lcyste ine NAC, v itam in E V itE, and py o lidine d ith ioca bam ate PDT C) in the eduction of H cyT - induced apoptos is. M ethods: F low cytom ete and aga- ose e lect opho esis of DNA f agmentation w e e applied to de tect and obse ve ce ll aopto sis. mRNA and p otein exp ession of caspase3 we e dete m ined by RT-PCR andW este n b lo tting. Results:H cyT cou ld induce ce ll apoptosis in a concent a tion-depend- en t manne . T he mRNA and p o te in exp ession of caspase3 w e e up- egulated in conco dancew ith the inc eased H cyT concent a- tion. NAC, V itE, o PDTC educedH cyT- indued apoptosis, concu ently dow n- egulated caspase3 exp ess ion. Conclusion:H c