D--鼠李糖β常春藤甙诱导乳腺癌细胞凋亡的机制研究的开题报告.docx

D--鼠李糖β常春藤甙诱导乳腺癌细胞凋亡的机制研究的开题报告

Title:

MechanismsofD-pinitol-β-ivyglycoside-inducedapoptosisinbreastcancercells

Introduction:

Breastcanceristhemostcommoncancerdiagnosedinwomenandaleadingcauseofcancer-relateddeathworldwide.Despiteadvancesintherapy,themortalityrateassociatedwiththisdiseaseremainshigh.Therefore,noveltherapeuticapproachesthatcanimprovesurvivalandqualityoflifeforpatientsareurgentlyneeded.Naturalcompoundsderivedfromplantshavegainedincreasingattentionaspotentialanticanceragentsduetotheirlowtoxicityandhighefficacy.D-pinitol-β-ivyglycoside(D-PIG)isolatedfromHederahelixisanaturalcompoundwithpotentanticancerproperties,includingtheinductionofapoptosisinbreastcancercells.However,theprecisemechanismsofD-PIG-inducedapoptosisinbreastcancercellsarenotfullyunderstood.TheobjectiveofthisstudyistoinvestigatethemolecularmechanismsunderlyingD-PIG-inducedapoptosisinbreastcancercells.

Methods:

ThehumanbreastcancercelllineMCF-7willbeusedinvitrotoinvestigatetheeffectsofD-PIGoncellviability,apoptosis,andrelatedmolecularmechanisms.Thefollowingexperimentalmethodswillbeperformed:

1.Cellviabilityassay:MCF-7cellswillbetreatedwithvariousconcentrationsofD-PIGfor24or48hours,andcellviabilitywillbemeasuredbyMTTassay.

2.Apoptosisassay:MCF-7cellswillbetreatedwithD-PIGfor24or48hours,andapoptosiswillbeevaluatedbyAnnexinV/PIstainingfollowedbyflowcytometryanalysis.

3.Westernblotanalysis:Theexpressionlevelsofvariousapoptosis-relatedproteins,suchascaspase-3,caspase-9,PARP,andBcl-2,willbeexaminedbywesternblotanalysis.

4.Real-timePCR:ThemRNAlevelsofvariousapoptosis-relatedgenes,suchascaspase-3,caspase-9,andBcl-2,willbequantifiedbyreal-timePCR.

Expectedresults:

WeexpectthatD-PIGwillinduceapoptosisinMCF-7cellsinadose-dependent