细胞冻存（Cell cryopreservation）.doc

细胞冻存（Cell cryopreservation） Cell cryopreservation directory Basic data: Background knowledge: Main content (steps): 1. Material Two, operation steps Three, matters needing attention Basic data: Background knowledge: Main content (steps): 1. Material Two, operation steps Three, matters needing attention Edit the basic data of this paragraph: 2 ml cell freeze tube Cell cryopreservation is one of the major methods for cell preservation. Using cryopreservation techniques, the cells are stored at -196 degrees C and stored in liquid nitrogen at low temperatures, allowing cells to temporarily escape their growth status and preserve their cell properties so that cells can be resuscitated for use as needed. Moreover, a moderate amount of cell preservation can prevent the cells from being contaminated or other unexpected events, and thus play a role in cell preservation. In addition, they can also be used to buy, send, exchange, and transport certain cells in the form of cell cryopreservation. Cryopreservation of cells when added to the medium protective agent -- 5%.15% final concentration of glycerol or two dimethyl sulfoxide (DMSO), the solution can crioscopic, coupled with slow freezing conditions, the moisture inside the cell with, reduce the formation of ice crystals, so as to avoid cell damage. The method of slow freezing and thawing can ensure cell survival. The standard freezing rate starts at -1 to -2 / min, and when the temperature is below -25 DEG C, it can be accelerated to -80 degrees Celsius and can be directly put into liquid nitrogen (-196 DEG C). Edit this paragraph of background knowledge: Cell culture passage and daily maintenance process, in training apparatus, medium and various preparations are needed to expend, and once the cells began to leave in the primary culture of various biological characteristics it will gradually change and change along with the increase in the number of passages and outer environmental conditions continue to have new change. T