星斑蛾蚋全线粒体基因组序列测定及分析.pdf

Chinese Journal of Applied Entomology 2017, 54(1): 3544. DOI: 10.7679/j.issn.20951353.2017.004 星斑蛾蚋全线粒体基因组序列测定及分析* ** *** 闫 俊 陈 汕 贾俊清 尤 平 710062 摘 要 【目的】 Psychoda alternata 【方法】DNA 【】 15 908 bptRNA rRNA A+T J N 37 39.6% A40.3% T11.8% C8.3% G A+T 79.9%【】 mtDNA J 9 N 4 13 26 关键词 Sequence analysis of the complete mitochondrial genome of Psychoda alternata ** *** YAN Jun CHEN Shan JIA Jun-Qing YOU Ping (College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China) Abstract [Objectives] Myiasis is a widely distributed kind of parasitic disease mainly caused by fly larvae. Psychoda alternata is one of the most common species of psychodidae that can trigger human myiasis. Determining the complete mitochondrial genome of P. alternata is valuable for general molecular and evolutionary studies on the Diptera, as well as for providing a theoretical basis for the prevention and treatment of myiasis. [Methods] DNA sequencing and cloning technology were used to obtain the complete mitochondrial genome sequence of P. alternata . [Results] The complete mitochondrial genome contained 15 908 nucleotides, consisting of protein coding genes, tRNA genes, rRNA genes, and a non-coding A+T-rich region. Two strands, the J and N strands, transcribed 37 genes. Genomic base composition was A 39.6%, T 40.3%, C 11.8%, G 8.3%, and A+T content comprised 79.9%. [C