浒苔多糖的磷酸化修饰工艺.pdf

※工艺技术 食品科学 2011, Vol. 32, No. 24 73 浒苔多糖的磷酸化修饰工艺 1 1,2 , 1 1 孙 雪 ，潘道东 * ，曾小群 ，曹锦轩 ( 1.宁波大学生命科学与生物工程学院，浙江 宁波 3 152 11； 2.南京师范大学 国家乳品加工技术研发分中心，江苏南京 2 10097) 摘 要：利用离子交换层析和凝胶层析对浒苔多糖进行分离纯化，并探讨其磷酸化修饰的最佳工艺条件。通过单因 素试验确定反应温度、反应时间、反应p H 值、磷酸化试剂质量浓度的取值范围，再用响应面设计法确定磷酸化修 饰的最佳条件。结果表明：反应时间、磷酸化试剂质量浓度显著影响浒苔多糖磷酸化程度，当反应温度8 0 ℃、反 应时间5h 、反应pH9.0 、磷酸化试剂质量浓度0.10g/mL 时，磷酸化修饰的浒苔多糖磷酸根含量( 以P 计)达到14 .40% 。 关键词：浒苔多糖；磷酸化；响应面法优化 Phosphorylation Modification of Polysaccharides from Entermorp ha 1 1,2, 1 1 SUN Xue ，PAN Dao-dong * ，ZEN G Xiao-qun ，CAO Jin-xu an (1. College of Life Science and Biotechnology, Ningbo University , Ningbo 3 152 11, China ； 2. Branch of National Dairy Processing Technology Developing Center, Nanj ing Normal University, Nanj ing 2 10097, China) Abstract ：This study was undertaken to optimize the phosphorylation of Enteromorp h polysaccharides prepared by hot water extraction, deproteinization , decolorization, and sequential chromatographic purification on DEA-Sepharose Fast Flow and Sephadex G- 100 columns. Phosphate group content in phosphorylated products was investigated with respect to reaction condition s including temperature, reaction time, pH and phosphorylation agent dosage (sodium tripolyphosphate-sodium trimetaphosphate at a mass ratio of 6 :1) by one-factor-at-a-time experiments and subsequent respon se surface analy sis. The optimal phosphorylation conditions were pH 9.0, temperature 80 ℃and phosphorylation agent dosage 0.10 g/mL for a reaction duration of 5 h. Under these condition s, the ph