电化学发光与酶免疫分析检测HBsAg的比较.pdf

DOI:10.13898/j.cnki.issn.1000-2200.2006.05.065

564JBengbuMedCol,lSeptember2006,Vo.l31,No.5

[]1000-2200(2006)05-0564-02·检验医学·

HBsAg

,

[]目的:(ECLIA)(ELISA)HBsAg。方法:HBsAg99

4[①HBsAg(+)/HBeAg(+)/HBcAb(+);②HBsAg(+)/HBeAb(+)/HBcAb(+);③HBsAg(+)/HBcAb

(+);④HBeAg(+)HBcAb(+)],ECLIAELISA、HBsAg。结果:ECLIACVELISA

、3.33.2,CV3.53.3,0.063、0.50.25ng/m。l①②③

3HBsAg100。④2ECLIA1∶50ELISA,ECLIAELISA。

①1∶50HBsAgCOI。结论:ELISAHBsAg,,

。Hook,。ECLIA,Hook,

。

[];;

[]R392.11[]A

ComparisonbetweenECLIAandELISAinmeasurementofHBsAg

HUANGQiu-fang,LIShou-rong

(LaboratoryDeparmtentofShanghaiEighthPeoplesHospita,lShanghai200235,China)

[Abstract]Objective:TocomparetheresultsofserumHBsAgdetectionbyeletrochemiluminescencemimunoassay(ECLIA)and

enzyme-linkedimmunobsorbentassay(ELISA).Methods:ECLIAandone-stepandtwo-stepELISAwereusedtodetectquantitative

HBsAgand99serumsampleswhichweredividedintofourmodel:s①HBsAg(+)/HBeAg(+)/HBcAb(+),②HBsAg(+)/HBeAb

(+)/HBcAb(+),③HBsAg(+)/HBcAb(+),④HBeAg(+)/HBcAb(+).Result:sThewithin-dayCVbyECLIAincreased

3.3and3.2comparedtobyone-stepELISAandtwo-stepELISAalone;andthebetween-dayCVbyECLIAincreased3.5and

3.3,andthesensitivitywas0.063,0.5and0.25ng/mlrespectively.ThepositiveratiobyECLIAandone-step,two-stepELISAwas

100inmode①l②③.Theresultwaspositiveintwosamplesofmode④lbyECLIA1∶50diluteandtwo-stepELISA,whiletheresult

wasnegativebyECLIAundilutedandone-stepELISA.Inmode①l,thelevelof1∶50dilutedHBsAgCOIwashigherthanthatof

undiluted.Conclusion:sOne-stepELISAissimpleandquickindetectingHBsAg,buttoohighortoolowconcentrationofthesample

mayissuefalsenegative.Toavoidhookeffec,ttwo-stepELISAisrecommended.ECLIAhasahighse