【2017年整理】固态发酵-水煮耦合炮制牛蒡子提高牛蒡子苷元含量.doc

固态发酵-水煮耦合炮制牛蒡子提高牛蒡子苷元含量 摘要:研究了黑曲霉Aspergillus nigerZJUT712菌株固态发酵和水煮工艺相耦合炮制牛蒡子.结果表明,该工艺提高了牛蒡 子中有效成分牛蒡子苷元的含量,从而有利于促进牛蒡子摄入体内迅速起效.最佳固态发酵炮制条件为: 015 g牛蒡子粉、3 g麸皮、2 g甘蔗渣、013 g蛋白胨和10 ml Mandels营养液,固液比1B316 g/ml,初始pH516, 30e发酵7 d.牛蒡子苷元产率 随底物初始浓度的增加而降低.A. nigerZJUT712转化01174 mmol/L牛蒡子苷的产率可达9310%. 7 d时间内A. niger ZJUT712水解牛蒡子苷的过程符合Monod方程,反应动力学常数为Vm=01083 7 mmol/(L#d),Km=0116 mmol/L. 关键词:牛蒡子苷;牛蒡子苷元;B-葡萄糖苷酶;中药;发酵;黑曲霉Aspergillus nigerZJUT712 中图分类号: O643/Q55 文献标识码: A Enhancement of Arctigenin Content by Processing of Fructus Arctii via Solid-State Fermentation Coupled with Poach OU Zhimin1, GUO Fangyuan1, YANG Gensheng1*, YAO Shanjing2 (1 Pharmaceuticals College, Zhejiang University of Technology, Hangzhou 310014 , Zhejiang, China; 2 Institute of Bioengineering, Zhejiang University, Hangzhou 310027 , Zhejiang, China) Abstract: The processing of fructus arctii, a Chinese medicine, by solid-state fermentation usingAspergillus nigerZJUT712 coupled with poach has been studied. The results show that this technique can significantly enhance the arctigenin content in fructus arctii. The optimum medium components are 015 g fructus arctii powder, 3 g bran, 2 g bagasse, 013 g peptone, and 10 ml Mandels nutr-i ent fluid. Solid-to-liquid ratio is 1B316 g/ml. Initial pH of the medium is 516. Lower yield was observed with higher initial arctiin concentration used. The yield reached 9310% when 01174 mmol/L arctiin was hydrolyzed at 30efor 7 d. In 7 d, the hydrolysis of arctiin is in accordance with the equation of Monod. The kinetic constants areVm=01083 7 mmol/(L#d) andKm=0116 mmol/L. Key words: arctiin; arctigenin;B-glucosidase; Chinese medicine; fermentation;Aspergillus nigerZJUT712 发酵中药是现代生物技术和中药研究的结合, 为中药的发展开辟了新空间[1].牛蒡子是菊科植物 牛蒡(Arctium LappaL.)的干燥成熟果实.牛蒡子 苷及牛蒡子苷元是牛蒡子发挥药效的重要成分,具 有利尿、泻下、降血压、抗癌抗突变、抗老年性痴呆和 治疗肾病综合症等作用[2~17].牛蒡子的药效与牛 蒡子苷在体内的代谢过程具有相关性,牛蒡子苷在 消化道中被肠道菌转变为苷元的脱甲基化合物,此 脱甲基化合物在肝脏儿茶酚-O-甲基转移酶的作用 下恢复为苷元,苷元被血液输送到各个器官发挥作 用[18].由于牛蒡子苷元可直接被人体吸收而发挥 药效,因此在体外将牛蒡子苷转化为牛蒡子苷元将 有助于促进牛蒡子摄入人体并快速起效.