LC-MS、MS法对血栓通注射液中皂苷成分快速定量分析 .pdf

LC-MS/MS法对血栓通注射液中皂苜成分快速定量分析

【摘要】目的：建立LC-MS/MS方法快速测定血栓通注射液中5种皂昔成分。方法：色谱柱BEHC18

(2.1mm*100mm,1.8pm),流速0.400mL/min,流动相乙睛-水，洗脱方式梯度洗脱，柱温30°C,进样量

2pL；采用多重反应监测模式(MRM)下负离子扫描方式，离子源ESI-。结果：人参皂昔Rg1、人参皂昔Rb1、人

参皂昔R、人参皂昔Rd及三七皂昔R1在浓度范围内线性关系良好，R20.999,定量限在0.34〜1.45ng/mL；低、中、

高3种浓度水平加样回收率在90.59%〜104.10%,相对标准偏差1.27%〜4.29%；进样精密度1.80%〜2.64%。

结论：本研究成功建立血栓通注射液中5种皂昔成分LC-MS/MS定量分析方法，方法操作简单，准确度和精密度良好，

并完成不同厂家不同批次血栓通注射液中5种皂昔成分测定。

【关键词】LC-MS/MS；血栓通注射液；人参皂昔Rg1；人参皂昔Rb1；人参皂昔R；人参皂昔Rd；三七皂昔

R1

LC-MS/MSmthodforrapidquantitativanalysisofsaponincomponntsinXushuantonginjction

ZHANGXiaojunl,FUHuihui2

1.GuangdongLiyunshangPharmacuticalCo.,Ltd,Yunfu,Guangdong527300,China；2.Yunfu

TraditionalChinsMdicinHospital,Yunfu,Guangdong527300,China

[Abstract]Objctiv：TostablishaLC-MS/MSmthodforrapiddtrminationoffivsaponincomponntsin

Xushuantonginjction.Mthods：ThchromatographiccolumnwasBEHC18(2.1mm*100mm,1.8pm),with

aflowratof0.400mL/min,thmobilphaswasactonitrilwatr,thlutionmthodwasgradintlution,th

columntmpraturwas30°C,andthinjctionvolumwas2pL；Adoptingthngativionscanningmthodundr

multiplractionmonitoringmod(MRM),andthionsourcwasESI-.Rsults：Thlinarrlationshipbtwn

ginsnosidRg1,ginsnosidRb1,ginsnosidR,ginsnosidRd,andnotoginsnosidR1wasgoodwithinth

concntrationrang,withR20.999,andthquantificationlimitsofthfivsaponincomponntswrfrom0.34to

1.45ng/mL；Thrcovryratsofthfivsaponincomponntsatlow,mdium,andhighconcntrationlvlsrangd

from90.59%to104.10%,a