小鹅瘟病毒卵黄抗体被动免疫期研究.pdf

学术研究 doi:10.3969/j.issn.1008-4754.2017.1.038 小鹅瘟病毒卵黄抗体被动免疫期研究 藏玉婷，王彬，宋扬，丁国杰* （哈药集团生物疫苗有限公司研发中心黑龙江哈尔滨 150069） ： ，琼 ， 摘要 本实验取3 批实验室试制的卵黄抗体 扩效价为1 ∶16，按0.5 mL/ 只接种1、3 日龄易感雏鹅 分别在注射抗体后 1、3、5 和7 日攻毒，观察攻毒保护情况。结果显示：1 日龄雏鹅注射抗体后 1 日攻毒 保护率为96.67% ，3 日攻毒保护率为100%，5 日攻毒保护率为86.67% ，7 日攻毒保护率为76.67% ；3 日龄 雏鹅注射抗体后 1 日攻毒保护率为100% ，3 日攻毒保护率为96.67% ，5 日攻毒保护率为86.67% ，7 日攻 毒保护率为80%。以免疫攻毒保护率不低于80%为标准，本研究确定小鹅瘟病毒卵黄抗体的被动免疫保 ， 。 护期为5 日 随着时间的延长，保护作用逐渐减弱 关键词：小鹅瘟病毒；卵黄抗体；雏鹅；被动免疫 ResearchofPassiveImmunityPeriodsofGooseParvovirusYolkAntibodies ZangYuting，WangBin，SongYang，DingGoujie* （HarbinPharmaceuticalGroupCoLtdBiologicalVaccine，Harbin 150069，China） Ab tract:3 batches of the goose parvovirus yolk antibodies were produced in laboratory with the titer of AGAR diffusion test 1:16. The experiment groups were inj ected with 0.5ml goose parvovirus yolk antibodies for goslings that were 1 and 3 days of age. After 1、3、5 and 7 days, the experiment groups and the control groups were both at- tacked with goose parvovirus. The results showed that for 1 day goslings, the virus attack protection was 96.67% af- ter 1 day, it was 100% after 3 days, it was 86.67% after 5 days, it was 76.67% after 7 days; and for 3 days goslings, the virus attack protection was 100% after 1 day, it was 96.67% after 3 days, it was 86.67% after 5 days, it was 80% after 7 days. In case of protective rate more than 80%, the research was determined that passive immunity period of goose parvovirus yolk antibodies was five days. As the extension of time, the protection of goslings was weakened. Keyword :Goose parvovirus; Yolk antibodies;