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用流式细胞仪分析脂肪细胞.ppt

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AnalysisandIsolationofAdipocytesbyFlowCytometryYangLI2016-04-05MajkaSM,MillerHL,HelmKM,etal.AnalysisandIsolationofAdipocytesbyFlowCytometry.Methodsinenzymology.2014;537:281-296.doi:10.1016/B978-0-12-411619-1.00015-X.

2BackgroundAimoftheresearchDifficultySolution

Majorcellsintheadiposetissue.Whatareadipocytes(fatcells)?

LipiddropletTwokindsofadipocytesinhuman

ProspectsofadipocytebiologyLipidstoreInhibiteatingIncreasemetabolismLeptin

ProspectsofadipocytebiologyAdipokinesInvolvedinenergymetabolismLipidstore

ProspectsofadipocytebiologySpecificallyfromadipose

tissueLipidstoreAdipokinesEnergymetabolism

Prospectsofadipocytebiology

Adipokinesplayansignificantroleinbiologicalfunction.

PrinciplediagramFlowCytometry(FCM)

Volume,Morphologicalcomplexityofcells,TotalDNA/RNAcontent,Apoptosis,Cellviability,............

Amethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.AnalysisFlowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)IsolationFluorescence-activatedcellsorting(FACS)

”AnalysisFlowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)BeckmanCoulter--MoFlo?XDP12345Amethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.Analysisrate:100,000events/sec.Sortrate:70,000events/sec.

AnalysisFlowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)QuantitiveHighresolutionimagingAmethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.

”Analysis1Flowcytometry(FCM)Confocallaserscanningmicroscope(CLSM)2Quantitive3Highresolutionimaging4Amethodforanalysisandisolationfree-floatingadipocytesbyFlowCytometry.5ImagingFlowCytometer(Amnis)Imagesforeverycell6

IsolationFluorescence-activatedcellsorting(FACS)Apassingcellcanbeselectivelyadddifferentcharge,fallingintodifferentcontainers.Amethodforanalysisandisolationfree-floatingadipocytesbyFlow

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