橙皮苷的高效液相色谱定量分析-Core.PDF

橙皮苷的高效液相色谱定量分析 1 2* 3 1 宁方刚 , 许建中 , 李小波, 陈清西 ( 1. , 361005; 2. , 361005; 3. , 361005) : : ( H PLC ) : H ypersil OD S2 , - - : 300 ~ 3000mg L , r09997; 600ng; 6 ( R SD ) 008% ; 9880% ~ 10 312% 4 8 , 170% ; 30 8 , 159% : , : ; ; ; ; ; : O 6577 : A : 1006- 2513( 2011) 02- - 04 Study on quantitative determ ination of hespe rid in by h igh pe rfo rmance liquid chromatography 1 2* 3 1 NING Fanggang, XU Jianzhong , LIXiaobo, CHEN Qingxi ( 1. Schoo l of L ife Sciences, X iam en Un iversity, X iam en 361005; 2. Th ird Institute of Oceanography State Ocean ic A dm inistrat ion, X iam en 36 1005; 3. Research and T eaching Center, Co llege of Chem istry Chem ica l Eng ineering, X iam en University, X iam en 361005) Abstract: O bjective: T o develop and va lidate a sensitive and spec if ic h igh perform ance liquid chroma tog raphy ( H PLC) m ethod for the determ ination of hesperidin. M ethod: H esper id in w as ana lyzed by a H ypers il ODS2 co lum n u s ing 16 84 ( v v) acetonitrile w ater as e luentw hich con tainede 006% acetic ac i