KLK6基因在宫颈癌中的表达及临床意义的开题报告.pdf
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KLK6基因在宫颈癌中的表达及临床意义的开题报告
Title:ExpressionandClinicalSignificanceofKLK6GeneinCervical
Cancer
Introduction:
Cervicalcancerisacommongynecologicalmalignanttumorthat
seriouslythreatenswomenshealth.Thepathogenesisandprogression
ofcervicalcancerarerelatedtovariousfactorssuchasgenetic
susceptibility,environmentalfactors,andHPVinfection.KLK6geneisa
memberofthekallikrein-relatedpeptidasefamily,whichisinvolvedin
thephysiologicalandpathologicalprocessesofvarioustissuesand
organsandhasbeenfoundtobehighlyexpressedinseveraltypesof
cancer.However,theexpressionandclinicalsignificanceofKLK6in
cervicalcancerremainsunknown.
Objectives:
Theaimofthisstudyistoinvestigatetheexpressionandclinical
significanceofKLK6geneincervicalcancer.
Methods:
Wewillcollect80cervicalcancertissuesamplesandpaired
adjacentnormaltissuesfrompatientswhowerediagnosedwithcervical
cancerandunderwentsurgicalresectionatourhospital.Theexpression
levelsofKLK6mRNAandproteinwillbedetectedbyquantitativereal-
timePCRandimmunohistochemistry,respectively.Wewillalsoanalyze
therelationshipbetweenKLK6expressionandclinicopathological
parameterssuchasage,tumorstage,lymphnodemetastasis,HPV
infection,andsurvivalstatusofpatients.
ExpectedResults:
WeexpecttofindthatKLK6expressionissignificantlyupregulated
incervicalcancertissuescomparedwithadjacentnormaltissues.
Furthermore,weanticipatethatKLK6overexpressionisassociatedwith
advancedtumorstage,lymphnodemetastasis,HPVinfection,andpoor
prognosis.
Conclusio